Comparison between Manual Blood Culture and Automated Blood Culture System in Cardiology Institute
DOI:
https://doi.org/10.61581/MJSP.VOL01/02/03Keywords:
Thyroid dysfunction,, Subclinical hypothyroid, Euthyroid, Blood culture, CardiologyAbstract
Objective: In this study we compared the two techniques used for detection of microorganisms.
Study Design: Retrospective cross-sectional.
Place and Duration: study was conducted at Choudhary Pervaiz Ellahi Institute of Cardiology, Multan from 2018 to 2019.
Methodology: Blood culture is gold standard for the detection of bacteremia. Two types of techniques are used for blood cultures which are the manual blood culture system (conventional) and the automated blood culture system (modern). We conducted 214 blood culture tests on manual blood culture system and 339 on automated blood culture system ( bact/alert).
Results: The percentage of positive yield was 10.3 with manual blood culture system and percentage of positive yield was 15.3 with bact/alert which is significantly higher.
Conclusion: This finding will contribute to reduction in mortality of patients with sepsis in our hospital and we recommend use of automated blood culture system over manual blood culture system at tertiary care hospitals.
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References
1. Wiens MO, Kumbakumba E, Kissoon N, Ansermino JM, Ndamira A, Larson CP. Pediatric sepsis in the developing world: challenges in dening sepsis and issues in post-discharge mortality. Clin Epidemiol 2012; 4: 319-25.
2. Çetin ES, Kaya S, Demirci M, Aridogan BC. Comparison of the BACTEC blood culture system versus conventional methods for culture of normally sterile body uids. Adv Ther 2007; 24: 1271-7.
3. Ahmad A, Iram S, Hussain S, Yusuf NW. Diagnosis of paediatric sepsis by automated blood culture system and conventional blood culture. J Pak Med Assoc. 2017 Feb;67(2):192- 195.
4. Elantamilan D. Comparative evaluation of conventional (manual) blood culture system and BacT/ALERT 3D (automated) blood culture system in a Tertiary care hospital. Sch J App Med Sci. 2017;5(2D):544-549.
5. Meyer J, Pare G. 2015. Telepathology impacts and implementation challenges: a scoping review. Arch Pathol Lab Med 139:1550–1557.
6. Smith KP, Kang AD, Kirby JE. Automated Interpretation of Blood Culture Gram Stains by Use of a Deep Convolutional Neural Network. J Clin Microbiol. 2018 Feb 22;56(3):e01521-17.
7. Broyer P, Perrot N, Rostaing H, Blaze J, Pinston F, Gervasi G, et al. An Automated Sample Preparation Instrument to Accelerate Positive Blood Cultures Microbial Identication by MALDI-TOF Mass Spectrometry (Vitek®MS). Front Microbiol. 2018 May 15;9:911.
8. Gopi A, Ambarish K, Shwethalatha N, Shree S, Ashwini K, Arpita S, et al. Time to positivity of microorganisms with BACTEC 9050: an 18- month study among children of 28 days to 60 months in an South Indian tertiary hospital. Int J Microbiol Res 2011; 2: 12-7.
9. Martines J, Paul VK, Bhutta ZA, Koblinsky M, Soucat A, Walker N, et al., Lancet Neonatal Survival Steering Team. Neonatal survival: a call for action. Lancet 2005; 365: 1189-97.
10. Seifert H, Wisplinghoff H. Bloodstream infection and endocarditis. Topley and Wilson's Microbiology and Microbial Infections. 2005.
11. Scholars Journal of Applied Medical Science ( SJAMS) Sch. J. App. Med Sci., 2017; 5(2D): 544-549.
12. Weinstein MP. Current blood culture methods and systems: clinical concepts, technology, and interpretation of results. Clinical Infectious Diseases. 1996 Jul 1; 23(1):40-6.
13. Hall KK, Lyman JA. Updated review of blood culture contamination. Clinical microbiology reviews. 2006 Oct 1; 19(4):788- 802.
14. Kirn TJ, Mirrett S, Reller LB, Weinstein MP. Controlled clinical comparison of BacT/alert FA plus and FN plus blood culture media with BacT/alert FA and FN blood culture media. Journal of clinical microbiology. 2014 Mar 1; 52(3):839.
15. Weinstein MP. Current blood culture methods and systems: clinical concepts, technology; and interpretation of results. Clin Infect Dis 1996; 23: 40– 46.
16. Rohner P, Pepey B, Auckenthaler R. Comparison of BacT/Alert with Signal blood culture system. Journal of clinical microbiology. 1995 Feb 1; 33(2):313-7.
17. Hasan AS, Uppal P, Arya S, Capoor MR, Nair D, Chellani H, Deb M, Aggarwal P. Comparison of BacT/Alert microbial detection system with conventional blood culture method in neonatal sepsis. Journal of Pediatric Infectious Diseases. 2008 Mar; 3(01):021-5.
18. Maria A, Phalgun S, Hans C, Dubey N, Goyal D. Comparison of conventional blood culture method and BacT/Alert system with respect to yield and time to positivity in neonata Sch. J. App. Med. Sci., Feb 2017; 5(2D):544-549.
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Copyright (c) 2020 Syed Khizar Abbas, Syed Jawad Hussain Shah
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